Journal: PLoS ONE

Article Title: Loss of Grainy Head-Like 1 Is Associated with Disruption of the Epidermal Barrier and Squamous Cell Carcinoma of the Skin

doi: 10.1371/journal.pone.0089247

Figure Lengend Snippet: (A) Blood levels of TSLP in Grhl1 +/+ (gray bar) and Grhl1 −/− mice (blue bar), measured with ELISA kit. (B) Levels of expression of antimicrobial peptides S100A8 and S100A9 in the epidermis of Grhl1 +/+ (gray bar) and Grhl1 −/− mice (blue bar), measured with Q-RT-PCR. (C) Representative skin sections of Grhl1 +/+ (top panel) and Grhl1 −/− mice (bottom panel) stained with toluidine blue to visualize dermal mast cells (purple cells). Scale bars represent 200 µm. (D) Quantification of skin infiltration with mast cells for Grhl1 +/+ (gray bar) and Grhl1 −/− mice (blue bar), estimated as numbers of stained cells per 1 mm 2 area of 10 µm thick skin section (using ImageJ software). (A, B, D) Significance (Student’s t-test, p-value) is shown above bars.

Article Snippet: The blood level of TSLP was measured using enzyme-linked immunosorbent assay (ELISA) kit: Quantikine Mouse TSLP (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions, on groups of seven Grhl1 −/− and five Grhl1 +/+ animals, at the age of about 4 months.

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Staining, Software

Journal: Journal of Inflammation Research

Article Title: Development of Predictive Models for Long-Term Endoscopic Response to Ustekinumab in Crohn’s Disease Based on Plasma Proteomics

doi: 10.2147/JIR.S563462

Figure Lengend Snippet: Differential expression of inflammation-related proteins between UST responders and non-responders.( A ) Volcano plot illustrating the differential expression of 92 inflammation-related proteins measured using the Olink ® platform. Red dots represent significantly upregulated proteins, blue dots indicate significantly downregulated proteins, and gray dots denote non-significant proteins. ( B ) Box-and-scatter plots showing the NPX of IL8, CD6, and TSLP in UST responders versus non-responders. ( C ) Heatmap depicting the expression profiles of the three DEPs across all participants. ( D ) ROC curve of IL8. ( E ) ROC curve of CD6. ( F ) ROC curve of TSLP.

Article Snippet: Commercial kits were used to measure interleukin-8 (IL8) (D8000C, R&D Systems, USA), CD6 (EH112RB, Thermo Fisher, USA), and thymic stromal lymphopoietin (TSLP) (DTSLP0, R&D Systems, USA).

Techniques: Quantitative Proteomics, Expressing

Journal: Journal of Inflammation Research

Article Title: Development of Predictive Models for Long-Term Endoscopic Response to Ustekinumab in Crohn’s Disease Based on Plasma Proteomics

doi: 10.2147/JIR.S563462

Figure Lengend Snippet: External ELISA validation and diagnostic performance of DEPs.( A ) Validation of plasma protein levels in an independent cohort of responders (n = 10) and non-responders (n = 10) using ELISA. Box plots show the distribution of IL8, CD6, and TSLP concentrations between groups. ( B ) ROC curves evaluating the diagnostic value of each biomarker.

Article Snippet: Commercial kits were used to measure interleukin-8 (IL8) (D8000C, R&D Systems, USA), CD6 (EH112RB, Thermo Fisher, USA), and thymic stromal lymphopoietin (TSLP) (DTSLP0, R&D Systems, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Biomarker Discovery, Diagnostic Assay, Clinical Proteomics

Journal: Journal of Inflammation Research

Article Title: Development of Predictive Models for Long-Term Endoscopic Response to Ustekinumab in Crohn’s Disease Based on Plasma Proteomics

doi: 10.2147/JIR.S563462

Figure Lengend Snippet: Performance and visualization of the logistic regression model for predicting response to UST.( A ) Variable importance plot of the multivariate logistic regression model based on three differentially expressed proteins (IL8, CD6, and TSLP).( B ) ROC curve of the model in the original dataset.( C ) Nomogram constructed based on the logistic regression model to facilitate individualized prediction of UST response.( D ) Calibration curve generated using 500 bootstrap resamples, assessing agreement between predicted and observed probabilities.

Article Snippet: Commercial kits were used to measure interleukin-8 (IL8) (D8000C, R&D Systems, USA), CD6 (EH112RB, Thermo Fisher, USA), and thymic stromal lymphopoietin (TSLP) (DTSLP0, R&D Systems, USA).

Techniques: Construct, Generated

Journal: Journal of Immunology Research

Article Title: Thymic Stromal Lymphopoietin Is Implicated in the Pathogenesis of Bullous Pemphigoid by Dendritic Cells

doi: 10.1155/2020/4594630

Figure Lengend Snippet: TSLP levels in sera and blister fluids of patients with BP were higher than those in controls. (a) The levels of TSLP in BP sera ( n = 35) were higher than those in HCs ( n = 44, p < 0.001). (b) The levels of TSLP in blister fluids of patients with BP ( n = 35) were higher compared to those of other blister dermatoses ( n = 39, p < 0.001). (c) The levels of TSLP in sera correlate with those in blister fluids ( n = 35, r s = 0.52, p = 0.0013). (d) The levels of TSLP in blister fluids were slightly higher than those in sera, but no significant differences were detected ( p = 0.089). TSLP levels in sera and blister fluids were investigated by enzyme-linked immunosorbent assay. p values were determined by the Wilcoxon rank test in (a, b) or the Wilcoxon signed rank test in (d). Correlation was determined by the Spearman rank correlation test in (c). A p value of less than 0.05 was considered statistically significant. Column scatter graphs show the median (lower quartile, upper quartile), and the measured values from individual patients were plotted by dots. TSLP: thymic stromal lymphopoietin; BP: bullous pemphigoid; HCs: healthy controls.

Article Snippet: TSLP levels in sera and blister fluids were measured using the Human TSLP Quantikine ELISA Kit (R&D Systems, Minneapolis, MN, USA), according to the manufacturer's recommendations.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Journal of Immunology Research

Article Title: Thymic Stromal Lymphopoietin Is Implicated in the Pathogenesis of Bullous Pemphigoid by Dendritic Cells

doi: 10.1155/2020/4594630

Figure Lengend Snippet: The expression of TSLP in the skin of patients with BP was higher compared to the HC skin. The TSLP expression in the skin of patients with BP and HCs was determined by immunohistochemistry. (a) Compared with the HC skin, the BP lesional skin showed a higher expression of TSLP mainly in the epidermis. (b) TSLP-positive cells (white arrowheads) in blisters of the BP lesional skin. (c) Number of TSLP-positive cells is greater in the BP lesional skin ( n = 10) compared to the HC skin ( n = 9, p = 0.0307). The number of each section is the average of counts calculated by four dermatologists in five randomly visual fields at HPF (0.068 mm 2 , ×400 magnification) of scanned sections. (d) Density of the TSLP expression in the epidermis of patients with BP was significantly higher than that in the HC epidermis (0.6379 vs 0.2019/mm 2 , p < 0.001). Density of the TSLP expression in the epidermis was scored by the ImageJ analysis system as described in the text. Results from three patients with BP and a HCs are shown in (a). Dashed white lines in (a, b) indicate the basement membrane zone or edge of blister. Asterisks ( ∗ ) indicate the area of blisters. Original magnification: ×100 and ×400. p values were determined by Student's t test in (c, d). A p value of less than 0.05 was considered statistically significant. Column scatter graphs show the mean ± standard deviation, and the measured values from individual patients were plotted by dots. TSLP: thymic stromal lymphopoietin; BP: bullous pemphigoid; HCs: healthy controls, HPF: high power field.

Article Snippet: TSLP levels in sera and blister fluids were measured using the Human TSLP Quantikine ELISA Kit (R&D Systems, Minneapolis, MN, USA), according to the manufacturer's recommendations.

Techniques: Expressing, Immunohistochemistry, Membrane, Standard Deviation

Journal: Journal of Immunology Research

Article Title: Thymic Stromal Lymphopoietin Is Implicated in the Pathogenesis of Bullous Pemphigoid by Dendritic Cells

doi: 10.1155/2020/4594630

Figure Lengend Snippet: The expression of TSLP in the BP lesional skin was associated with the amount of DC-SIGN-positive DCs, but not LCs. (a) Staining with DC-SIGN (green), TSLP (red), and DAPI (blue) showed the amount of DC-SIGN-positive DCs (white arrowheads) infiltrated in the dermis beneath blisters or TSLP-positive epidermis. (b) Staining with langerin (green), TSLP (red), and DAPI (blue) showed that the LCs (white arrowhead) existed in the epidermis regardless of whether TSLP was positive or not. Results from three patients with BP are shown in (a, b), respectively. Dashed white lines indicate the basement membrane zone or edge of blister. Asterisks ( ∗ ) indicate the area of blisters. Original magnification: ×100 and ×200, white scale bar = 100 μ m. BP: bullous pemphigoid; DAPI: 4,6-diamino-2-phenyl indole; DCs: dendritic cells; DC-SIGN: dendritic cell–specific intercellular adhesion molecule-3-grabbing nonintegrin; LCs: Langerhans cells; TSLP: thymic stromal lymphopoietin.

Article Snippet: TSLP levels in sera and blister fluids were measured using the Human TSLP Quantikine ELISA Kit (R&D Systems, Minneapolis, MN, USA), according to the manufacturer's recommendations.

Techniques: Expressing, Staining, Membrane

Journal: Heliyon

Article Title: Anti-atopic dermatitis effect of fraxinellone via inhibiting IL-31 in vivo and in vitro

doi: 10.1016/j.heliyon.2024.e35391

Figure Lengend Snippet: Fraxinellone inhibits the expression of inflammatory cytokines in the lesion skin and serum of DNFB-induced AD-like mice. The levels of IL-31(A), IL-4 (B), and TSLP (C) in the serum of AD mice were measured by ELISA kit. The levels of IL-31(D), IL-4 (E), and TSLP (F) in the lesion skin in each group ( *P < 0.05, **P < 0.01, ***P < 0.001, versus DNFB group; # P < 0.05, ## P < 0.01, ### P < 0.001, comparison between groups; N.S, not significant; n = 4).

Article Snippet: A hematoxylin and eosin (H&E) staining kit (cat: G1120, Solarbio), mouse IL-31 enzyme-linked immunosorbent assay (ELISA) kit (cat: EK1100, Boster), mouse IL-4 ELISA kit (cat: TW10973, Tongwei), mouse Thymic stromal lymphopoietin (TSLP) ELISA kit (cat: E-EL-M0646, Elabscience) were used.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Comparison

Journal: Mucosal immunology

Article Title: Critical role of TSLP-responsive mucosal dendritic cells in the induction of nasal antigen-specific IgA response.

doi: 10.1038/mi.2016.103

Figure Lengend Snippet: Figure 2 TSLP and TSLPR expression in DCs of nasally immunized mice. Mice were nasally immunized weekly for 3 consecutive weeks with PspA only, CT only, or PspA plus CT. All the samples were harvested at 1 week after the last immunization. (a) RT-qPCR analysis of TSLP mRNA expression in CD11c þ DCs from spleen, CLN, NALT, NP, and lung tissue of nasally immunized mice. CD11c þ DCs were isolated by MACS and pooled from three mice per group. The mRNA expression level was normalized to that of b actin, and data are expressed relative to the value in PspA only DCs. Values represent the mean±s.e.m. of values from three independent experiments. **Po0.01 vs. PspA only DCs. (b) Flow cytometric analysis of TSLPR expression in CD11b þCD11c þ DCs from spleen, NP, lung tissue of nasally immunized mice (open histogram). Shaded histograms indicate isotype control. MFI indicating TSLPR expression is shown. Values are means±s.e.m. (n ¼ 3 per group). Data are representative of three independent experiments. *Po0.05 vs. mice nasally immunized with PspA plus CT. CLN, cervical lymph node; CT, cholera toxin; DC, dendritic cells; MACS, magnetic-activated cell sorting; MFI, mean fluorescence intensity; mRNA,messenger RNA; NP,nasal passages; NS, not significant; PspA, pneumococcal surface protein A; RT- qPCR, real-time-quantitative PCR; TSLP, thymic stromal lymphopoietin.

Article Snippet: The concentration of TSLP in broncho-alveolar fluid samples was measured by using a TSLP ELISA kit (R&D Systems, Minneapolis, MN, USA).

Techniques: Expressing, Quantitative RT-PCR, Isolation, Control, FACS, Fluorescence, Real-time Polymerase Chain Reaction